Dr. Ing. Petr Behenský: “BLOG PHYTENEO”

Resorption of Herbal Agents into the Human Organism

11. October 2016 - Dr. ing. Petr Behenský
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Resorption of Herbal Agents into the Human Organism

Within the scope of our research activities, we dealt with the following question – to what extent resorption of herbal agents in Phyteneo Kolodium forte take place in the human organism.  The subject of our interest was the native raw plant, Thuya occidentalis and the terminal branches and stem of the dried plant, Chelidonium majus. Since there is no relevant data about the amount of resorption of the above stated agents to date, we have conducted a research project in cooperation with physicians and the Faculty of Natural Science at the Palacky University in Olomouc, related to this issue. Below, you will find a summary of the results of this project.

Screening of Active Agents
Firstly, it was necessary to conduct a screening of the active agents in plant drugs with the aim being to identify and consequently also quantify the analytes, which (1) form 90% of the overall area of response when using the corresponding methods beginning with the largest contribution, (2) its response demonstrates more than 5% of the overall area of analytes and (3) if toxicology characteristics may be assumed for the analytes, meaning that the analyte in identifiable based on information from the spectra library and there is information available about the toxicology or pharmacology effects or it can for example be assumed that it belongs to the group of agents, with information available about the toxicology or pharmacology effects based on the mass of the spectra.

For the Thuya occidentalis drug, screening was conducted using the GC-MS and also supplement by HPLC-MS. The results are illustrated in Fig. 1. This is an overview of the results acquired using GC-MS. According to the assumed HPLC technique, it identified analytes, which were not relevant from the point of view of using the agent and the resorption study. The share of the overall response of analytes is stated as an average of 5 parallel measurements from various plant material samples. The analytes in bold were selected as quality markers of the herbal agents. Methyl sugars and glycosides were not selected for further measurements because it can be assumed that they are neither significant from the point of view of pharmacology nor from the point of view of their potential toxicity. Therefore, their further identification was not clarified. However, the analytes, beta-phellandrene and fenchone, were included in further measurements even though their relative response was lower than the 5% criteria. The analyte, pinene was also included in further measurements. Pinene is brought into the agent via another additive agent, which is purified pine oil (Terebinthinae etheroleum rectificatum).

For the Chelidonium majus drug, screening was conducted using the HPLC-MS technique and also supplemented by GC-MS. The results are illustrated in Fig. 2. This is an overview of the results acquired using HPLC-MS. According to the assumption, the GC-MS technique did not identify any relevant analytes. The share of the overall response of analytes is stated as an average of 5 parallel measurements from various plant material samples.
The analytes in bold were selected as quality markers of the herbal agents. Only the analyte, O- glutarylcarnitine was not selected for further measurements because it is not neither significant from the point of view of pharmacology nor from the point of view of their potential toxicity. However, the analytes, protropine, berberine, sanguinarine and chelerythrine were included in further measurements even though their relative response was lower than the 5% criteria.

Resorption Tests
The resorption of individual analytes, resp. the active agents were studied in Kolodium forte. Generally, a minimal resorption for this atopic applied product is desirable. This means that the treatment interfered into the organism only locally, where the product is used and system exposure was as low as possible.

Resorption was defined under the conditions, which correspond with a one-time application in the amount of 0.2ml of the product.

Fig. 3. illustrates an overview of the extent of resportion of agents when Kolodium forte is applied. The value of the extent of resorption is an average from 12 skin samples. The resorption value is expressed as a percentage of the ration of analyte concentration in the accepted solution (so, the quantity, which was absorbed by the skin) compared to the analyte concentration on that side of the skin, where Kolodium forte was applied.

Fig. 4. illustrates the extent of resorption of agents when Kolodium forte is applied. The value of the extent of resorption is an average from 12 skin samples. The resorption value is expressed as an absolute quantity of individual agents, which, with regard to the extent of resporption (see Tab. 4), would be absorbed by the skin in one average application (0.2ml) of Kolodium forte.

Conclusion
From the results, it is clear that the extent of resorption ranges on a low level up to 10%, except for the analyte, protropin, where the resorption value significantly diverts up to a level of about 26%. From the system exposure of the organism point of view, the quantity of agents, which are absorbed by the skin to the organism during the application of the product, is on a very low level. This, practically insignificant extent of system exposure is caused by (1) an application performed according to the Instructions for Use of the product applied in a relatively small quantity of the product, (2) concentration of the monitored analytes in the product is relatively low and (3) the extent of resorption is relatively very low.

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